Preparation and Characteristic of Carbofuran Molecularly Imprinted Polymer Microspheres for Solid Phase Extraction

Uniform sized carbofuran molecularly imprinted polymer microspheres （MIPMs） were prepared by one step swelling method using azobisisobutyronitrile as initiator, 2—3 μm polystyrene microspheres as seed, carbofuran as template, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross linker. The Scanning Electron Microscope （SEM）, equilibrium binding experiment and competitive adsorption experiment were used to study MIPMs’ morphology, binding ability and adsorption selectivity. Enrichment effects of MISPE and C18 SPE were also compared. Results showed that the maximum binding capacity of carbofuran MIPMs was 25.94 mg/g within 90 min; these carbofuran microspheres could adsorb carbofuran selectively when carbofuran, methomyl and 3 hydroxycarbofuran coexisted in water. Compared to C18 SPE’s recovery, the carbofuran MISPE’s recovery of samples spiked with carbofuran was above 85% after using six times. It could be used to enrich carbofuran in water sample.