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    WANG Kangjing, LI Ting, DING Zhongyang, DONG Weifu. Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme[J]. Journal of Functional Polymers, 2023, 36(4): 392-398. doi: 10.14133/j.cnki.1008-9357.20230208001
    Citation: WANG Kangjing, LI Ting, DING Zhongyang, DONG Weifu. Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme[J]. Journal of Functional Polymers, 2023, 36(4): 392-398. doi: 10.14133/j.cnki.1008-9357.20230208001

    Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme

    • Through self-stable precipitation polymerization, styrene (St), maleic anhydride (MA), and glycidyl methacrylate (GMA) were copolymerized to obtain nanoparticles with regular morphology and uniform particle size. This one-pot method has no stabilizer and emulsifier, and requires no stirring. The post-treatment is simple, and the product can be obtained only by centrifugation. The effects of monomer feed ratio and solvent on the morphology and chemical composition of nanoparticles were studied by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The prepared nanoparticles can be used as emulsifiers to stabilize Pickering emulsion and immobilize lipase through epoxy groups. It was verified that lipase was anchored on the oil-water interface by immobilizing on the particles. The influence of the contact angle of particles and the solvent volume ratio of n-Heptane to water (RO/W) on the emulsion size was observed by fluorescence microscope. With the increase of contact angle and RO/W, the emulsion size decreases, which is also beneficial to the enzymatic reaction. The effects of nanoparticle mass content, lipase initial mass concentration, RO/W on the size of Pickering emulsion and subsequent enzyme catalyzed reaction were investigated. At the condition of n(GMA)∶n(St)∶n(MA) = 2∶1∶1, the mass content of nanoparticles 0.5%, the mass concentration of lipase 3 mg/mL, and RO/W 5∶5, the specific activity of immobilized enzyme was the highest, which was up to 7.5 times that of free enzyme, and it showed excellent reusability.
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