Advanced Search

    ZHA Li, WANG Luyao, ZHENG Yahui, CHE Jianfei. Biomineralization and Surface Functionalization of Cellulose/Sodium Alginate Composite Aerogels[J]. Journal of Functional Polymers, 2020, 33(4): 382-389. doi: 10.14133/j.cnki.1008-9357.20191120001
    Citation: ZHA Li, WANG Luyao, ZHENG Yahui, CHE Jianfei. Biomineralization and Surface Functionalization of Cellulose/Sodium Alginate Composite Aerogels[J]. Journal of Functional Polymers, 2020, 33(4): 382-389. doi: 10.14133/j.cnki.1008-9357.20191120001

    Biomineralization and Surface Functionalization of Cellulose/Sodium Alginate Composite Aerogels

    • Cellulose/sodium alginate (Cellu/SA) composite aerogels were prepared via freeze-drying technique as a valid method to develop 3D scaffolds for tissue engineering. Cellulose with hydroxyl groups showed weak apatite nucleation ability in simulated body fluid(SBF). In order to induce the formation of apatite crystal, oxidation modification of Cellu/SA composite aerogels was carried out with sodium periodate and sodium chlorite. The hydroxyl groups at C2 and C3 of Cellulose and SA were oxidized into carboxyl groups, leading to improve mineralization ability. The morphology and structure were characterized by Fourier Transform Infrared (FT-IR) spectrometer, Scan Electron Microscope (SEM), X-Ray Diffraction (XRD) and X-ray photoelectron spectroscopy. Results show that the formation of HA on the surface of dicarboxylic Cellu/SA composite aerogels is faster with smaller grain size, and the deposition layer is more uniform after mineralization for 7 d. The Cellu/SA composite aerogels thus possess an ultrafine 3D nanoporous network structure. It is noticeable that the dicarboxylic Cellu/SA composite aerogel is a promising candidate for orthopedic biomaterial application with a Ca/P molar ratio of 1.35. In addition, the aerogel has good biocompatibility demonstrated by CCK8 assay and Live/Dead fluorescence staining of L929 cells. The L929 cells activity of both Cellu/SA and dicarboxylic Cellu/SA composite aerogels extracts are higher than 80%. When the culture duration is prolonged to 72 h, an obvious increase in the intensity of green fluorescence is observed, suggesting that the number of cells is increased. The biocompatible mineralized dicarboxylic Cellu/SA composite aerogels can promote cell growth and differentiation and are potential to be applied as a bone repair material.
    • loading

    Catalog

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return