Abstract: Azobenzene-containing chiral liquid crystal polymers (P(S)A-(CH₂)₆-Azo and P(S)A-(CH₂)₁₀-Azo-C₄H₉) with a polyacetylene main chain were designed and synthesized by Rh(nbd)Cl₂-catalyzed homopolymerization. The chiral carbon was directly connected to the main chain, while the azobenzene group was linked through ether spacer. The polyacetylene backbone provided the rigidity, while the azobenzene mesogen on the side chain provided the photosensitivity and the ability for ordered arrangement. The liquid crystal properties of the two chiral liquid crystal polymers were regulated by introducing spacer groups and azobenzene end groups of different lengths and adjusting the distance between chiral carbon and azobenzene group. The photoisomerization properties of chiral liquid crystal polymers in tetrahydrofuran(THF) were measured using ultraviolet-visible(UV-Vis) absorption spectra, and the changes in chiral signals were characterized by circular dichroism spectra. The chiral signals did not change under UV and blue light irradiation, suggesting that the helical arrangement of the main chain and expression of chirality were not affected by the photoisomerization. The liquid crystal properties, photoisomerization, and chirality of films based on chiral liquid crystal polymer were also studied. Only a P(S)A-(CH₂)₁₀-Azo-C₄H₉ film exhibited a cholesteric phase. The chiral signals of films based on chiral liquid crystal polymer could be controlled by the irradiation of UV light and blue light.