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    王康静, 李 婷, 丁重阳, 东为富. 共聚微球稳定皮克林乳液的研究及酶固定化应用[J]. 功能高分子学报,2023,36(4):392-398. doi: 10.14133/j.cnki.1008-9357.20230208001
    引用本文: 王康静, 李 婷, 丁重阳, 东为富. 共聚微球稳定皮克林乳液的研究及酶固定化应用[J]. 功能高分子学报,2023,36(4):392-398. doi: 10.14133/j.cnki.1008-9357.20230208001
    WANG Kangjing, LI Ting, DING Zhongyang, DONG Weifu. Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme[J]. Journal of Functional Polymers, 2023, 36(4): 392-398. doi: 10.14133/j.cnki.1008-9357.20230208001
    Citation: WANG Kangjing, LI Ting, DING Zhongyang, DONG Weifu. Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme[J]. Journal of Functional Polymers, 2023, 36(4): 392-398. doi: 10.14133/j.cnki.1008-9357.20230208001

    共聚微球稳定皮克林乳液的研究及酶固定化应用

    Stabilization of Pickering Emulsion by Terpolymer Microspheres and Application of Immobilized Enzyme

    • 摘要: 以苯乙烯(St)、马来酸酐(MA)、甲基丙烯酸缩水甘油酯(GMA)为单体,通过自稳定沉淀聚合反应制备了带有环氧基团的形貌规整、粒径均一的纳米粒子(PMG),该纳米粒子可作为乳化剂稳定皮克林乳液,并利用环氧基团来固定化脂肪酶。系统研究了单体投料比、溶剂对纳米粒子形貌和化学组成的影响,以及纳米粒子添加量、脂肪酶质量浓度、溶剂对皮克林乳液粒径和酶催化反应的影响。结果表明,当n(GMA)∶n(St)∶n(MA)为2∶1∶1、纳米粒子质量分数为0.5%、脂肪酶初始质量浓度为3 mg/mL、正庚烷与去离子水体积比为5∶5时,固定化脂肪酶的催化比活性最高,是游离酶催化比活性的7.5倍,且具有优良的重复使用性能。

       

      Abstract: Through self-stable precipitation polymerization, styrene (St), maleic anhydride (MA), and glycidyl methacrylate (GMA) were copolymerized to obtain nanoparticles with regular morphology and uniform particle size. This one-pot method has no stabilizer and emulsifier, and requires no stirring. The post-treatment is simple, and the product can be obtained only by centrifugation. The effects of monomer feed ratio and solvent on the morphology and chemical composition of nanoparticles were studied by Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). The prepared nanoparticles can be used as emulsifiers to stabilize Pickering emulsion and immobilize lipase through epoxy groups. It was verified that lipase was anchored on the oil-water interface by immobilizing on the particles. The influence of the contact angle of particles and the solvent volume ratio of n-Heptane to water (RO/W) on the emulsion size was observed by fluorescence microscope. With the increase of contact angle and RO/W, the emulsion size decreases, which is also beneficial to the enzymatic reaction. The effects of nanoparticle mass content, lipase initial mass concentration, RO/W on the size of Pickering emulsion and subsequent enzyme catalyzed reaction were investigated. At the condition of n(GMA)∶n(St)∶n(MA) = 2∶1∶1, the mass content of nanoparticles 0.5%, the mass concentration of lipase 3 mg/mL, and RO/W 5∶5, the specific activity of immobilized enzyme was the highest, which was up to 7.5 times that of free enzyme, and it showed excellent reusability.

       

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