NO释放性抗菌、抗氧化水凝胶的制备及性能

尚亚廷; 李世超; 周莹杰; 郭江娜; 严锋

doi:10.14133/j.cnki.1008-9357.20230207001

NO释放性抗菌、抗氧化水凝胶的制备及性能

doi: 10.14133/j.cnki.1008-9357.20230207001

尚亚廷^1,,
李世超¹,
周莹杰¹,
郭江娜^2, ,,
严锋^{1, 2, ,}

1.
东华大学材料科学与工程学院, 纤维材料改性国家重点实验室, 上海 201620
2.
苏州大学材料与化工学部, 江苏省新型功能高分子材料工程实验室, 江苏苏州 215123

基金项目: 国家自然科学基金（21835005, U1862109和22005045）；中央高效基本科研业务费专项资金项目（2232020 D-07）

详细信息

作者简介:
尚亚廷（1998—），女，硕士研究生，主要研究方向为生物医用材料。E-mail：shangyt0504@163.com

通讯作者:
郭江娜，E-mail: guojn@suda.edu.cn

严　锋，E-mail：fyan@suda.edu.cn

中图分类号: O69
计量
- 文章访问数: 36
- HTML全文浏览量: 8
- PDF下载量: 13
- 被引次数: 0
出版历程
- 收稿日期: 2023-02-07
- 录用日期: 2023-04-10
- 网络出版日期: 2023-04-15

Preparation and Properties of Antibacterial and Antioxidant NO Releasing Hydrogels

SHANG Yating^1
,,
LI Shichao¹,
ZHOU Yingjie¹,
GUO Jingna^{2
, ,},
YAN Feng^{1, 2
, ,}

1.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China
2.
Jiangsu Engineering Laboratory of Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, Jiangsu, China

摘要

摘要: 设计合成了具有光热和NO控释放性的抗菌、抗氧化水凝胶。利用Fe³⁺和戊二醛将聚赖氨酸、单宁酸交联形成水凝胶，并负载NO供体分子S-亚硝基-N-乙酰基-DL-青霉胺获得兼具光热和NO控释功能的水凝胶（NO-Fe_x-TA₂₀, x = 5, 7, 9）。所合成水凝胶具有良好的光热性能，在1 W/cm²近红外光照射下10 min内温度可达到43.8 ℃。在可见光和37 °C恒温下，水凝胶3 h累计释放的NO达到36.48 µM。抗菌实验表明，水凝胶对金黄色葡萄球菌具有优异的杀菌活性，杀菌活性大于99.9%；同时，其能够高效清除2,2-联苯基-1-苦基胼基（DPPH）自由基，清除率大于84.3%，具有显著的抗氧化效果。
- 抗菌 /
- 抗氧化 /
- 一氧化氮 /
- 聚赖氨酸 /
- 水凝胶
Abstract: Bacterial infection seriously endangers patients' health. Thus, it is imperative to develop highly efficient antibacterial materials. In this study, antibacterial and antioxidant hydrogel with photothermal effect and nitric oxide (NO) gas-releasing property was designed and synthesized. The hydrogel was formed by crosslinking ε-polylysine and tannic acid using Fe³⁺ and glutaraldehyde and loading NO donor S-nitroso-N-acetyl-DL penicillamine (SNAP). The NO-Fe₉-TA₂₀ hydrogel shows high photothermal effect and the temperature of hydrogel can reach 43.8 ℃ within 10 minutes under 1 W/cm² near-infrared irradiation. Additionally, the NO-Fe₉-TA₂₀ hydrogel shows effective NO-release activities. Under visible light and at 37 ℃, the amount of NO released from the hydrogel reached 36.48 μM within 3 h. Benefitting from the synergetic effect of cations of ε-poly-L-lysine (EPL) and the released NO, NO-Fe₉-TA₂₀ hydrogel exhibits antibacterial activity (> 99.9%) towards Staphylococcus aureus, and can effectively scavenge 1,1-dipheny-2-picrylhydrazyl (DPPH) free radicals (> 84.3%), which may protect cells from oxidative stress damage. This study may provide a new approach for bacterial infections treatment.
- antibacterial /
- antioxidant /
- nitric oxide /
- ε-polylysine /
- hydrogel

HTML全文

图 1 SNAP的(a) ¹H-NMR 图谱与(b)紫外吸收光谱

Figure 1. (a) ¹H-NMR (b) UV-Vis absorption spectra of SNAP

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图 2 Fe₉-TA₂₀水凝胶的 (a)红外谱图与(b)流变曲线

Figure 2. (a) FT-IR and (b) frequency sweep test of Fe₉-TA₂₀ hydrogel

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图 3 (a) Fe_x-TA₂₀水凝胶在NIR光照射下的温度曲线; (b) Fe₉-TA₂₀水凝胶在不同功率 NIR 照射下的温度曲线; (c) Fe₉-TA₂₀水凝胶的加热-冷却曲线; (d) NIR照射Fe₉-TA₂₀水凝胶的热红外图

Figure 3. (a) Temperature curves of Fe_x-TA₂₀ hydrogels under NIR irradiation; (b) Temperature curves of Fe₉-TA₂₀ hydrogel under NIR irradiation with different irradiation power; (c) Heating-cooling curve of Fe₉-TA₂₀ hydrogel; (d) Thermal infrared images of Fe₉-TA₂₀ hydrogel irradiated by NIR

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图 4 水凝胶的抗氧化性能测试: (a) DPPH自由基清除率; (b) ABTS法综合抗氧化能力

Figure 4. Test of antioxidant properties of hydrogel; (a) Scavenging rate of DPPH free radicals; (b) Overall antioxidant capacity of ABTS method

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图 5 NO-Fe₉-TA₂₀水凝胶的(a) NO释放标准曲线和(b) 在不同温度下的NO释放曲线

Figure 5. (a) Standard curve of NO release and (b) NO release curve of at different temperatures of NO-Fe₉-TA₂₀ hydrogel

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图 6 NO- Fe₉-TA₂₀ 水凝胶的抗菌性能

Figure 6. Antibacterial properties of NO- Fe₉-TA₂₀ hydrogel

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