强韧支架用丝素蛋白基生物墨水及其3D打印支架模拟软件的开发

耿亚楠; 赵梦露; 姚响; 张耀鹏

doi:10.14133/j.cnki.1008-9357.20221107001

强韧支架用丝素蛋白基生物墨水及其3D打印支架模拟软件的开发

doi: 10.14133/j.cnki.1008-9357.20221107001

东华大学材料科学与工程学院, 纤维材料改性国家重点实验室, 上海 201620

基金项目: 上海市科学技术委员会（20XD1400100、22520711900、20ZR1402400）；国家自然科学基金（52273125、52173031）

详细信息

作者简介:
耿亚楠（1998—），女，硕士，主要研究方向为生物墨水及其3D打印。E-mail：13262588980@163.com

通讯作者:
姚　响，E-mail：yaoxiang@dhu.edu.cn；张耀鹏，E-mail：zyp@dhu.edu.cn

中图分类号: R318.08
计量
- 文章访问数: 110
- HTML全文浏览量: 34
- PDF下载量: 106
- 被引次数: 0
出版历程
- 收稿日期: 2022-11-07
- 录用日期: 2022-12-30
- 网络出版日期: 2023-01-04
- 刊出日期: 2023-04-01

Development of Silk Fibroin Based Bio-Ink for Tough Scaffold and Related Simulation Software for 3D Printing Scaffold

State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China

摘要

摘要: 基于丝素蛋白（SF）和有限元分析方法，开发了一种强韧支架用丝素蛋白基生物墨水及其3D打印支架压缩性能模拟软件。表征了墨水的可打印性及对应水凝胶和3D打印支架的力学性能，评估了相关打印支架的细胞相容性。基于所述丝素蛋白基生物墨水，设计并制备了不同高度和孔隙率的3D打印支架，利用所开发软件和电子万能材料试验机对打印支架的压缩性能进行了模拟预测和实测对比。结果表明：该墨水可打印性佳，对应支架强度高韧性好、细胞相容性好。所开发软件操作简便，具有3D打印支架建模、模型压缩力学性能预测以及指导3D打印支架快速制备等功能。
- 丝素蛋白 /
- 3D打印 /
- 软件开发 /
- 力学性能模拟 /
- 支架
Abstract: At present, the existing scaffold materials cannot well meet the high strength and toughness requirements for the repairing of typical stressed tissues. And the commercial software for 3D printing scaffold modeling and mechanical performance simulation is complex to operate and has poor interoperability with 3D printers. In order to solve these problems, a silk fibroin (SF) based bio-ink was prepared for tough scaffold, and a software was developed for compression performance simulation of 3D printing scaffold based on SF and finite element analysis strategy. The printability of the bio-ink and the mechanical properties of the corresponding hydrogels and 3D printing scaffolds were characterized. The cell compatibility of the related scaffolds was evaluated by using fibroblasts and living/dead staining kit. Based on the SF based bio-ink, scaffolds with different heights and porosities were designed and printed. By using the developed software and the universal testing machine, the compression performance of related scaffolds was simulated and tested separately, and further compared with each other. Results show that the SF based bio-ink has good printability. And the 3D printing scaffold prepared by this ink presents high strength and toughness (modulus of elasticity: (1.86 ± 0.28)MPa, modulus of compression: (1.95 ± 0.11)MPa, elongation at break: (114.03 ± 14.40)%, compression strain ≥ 70%). In addition, the scaffold has good cell compatibility (L929 cell viability≥92.4%), which is expected to be used for the repair and regeneration of stressed tissues. The software is easy to operate, and can be used not only for the modeling of 3D printing scaffolds, but also for accurately predicting their compressive mechanical properties. In addition, the model data can also be directly imported into the 3D printer to guide the efficient preparation of the corresponding 3D printing scaffolds. This research is expected to provide important guidance for the rapid design and fabrication of tissue specific 3D printing scaffolds with bionic structure and mechanical properties.
- silk fibroin /
- 3D printing /
- software development /
- mechanical property simulation /
- scaffold

HTML全文

图 1 开发软件的核心功能：（a）3D打印支架建模；（b）支架压缩性能模拟分析；（c）模型数据导出与3D打印机共享

Figure 1. Core functionalities of the developed software: (a) Modelling of 3D printed scaffold; (b) Simulation analysis of scaffold compression performance; (c) Model data sharing with the 3D printer

下载: 全尺寸图片幻灯片

图 2 利用RSF/AM/OBC墨水制备的典型3DS的力学性能：（a）拉伸应力-应变曲线；（b）压缩应力-应变曲线

Figure 2. Mechanical properties of typical 3DS prepared using RSF/AM/OBC bio-ink: (a) Tensile stress-strain curve; (b) Compressive stress-strain curve

下载: 全尺寸图片幻灯片

图 3 L929细胞在RSF/AM/OBC打印支架上培养7 d后的活/死荧光染色图

Figure 3. Live/dead staining of the cells on the RSF/AM/OBC printing scaffold after cultured 7 d

下载: 全尺寸图片幻灯片

图 4 3DS建模及模型数据导出指导3DS的快速制备

Figure 4. 3DS modeling and its guidance for the rapid preparation of corresponding 3DS

下载: 全尺寸图片幻灯片

图 5 不同高度、不同孔隙率支架压缩应力-应变曲线真实测量值和模拟值的对比结果

Figure 5. Comparison of real measured and simulated values of compressive stress-strain curves of scaffolds with different heights and porosities

下载: 全尺寸图片幻灯片

表 1 丝素蛋白基生物墨水的配方

Table 1. Specific formulations of the silk fibroin based bio-inks

Sample	m（RSF）/g	m（AM）/g	m（OBC）/g	m（Ru）/mg	m（SPS）/mg	m（MBA）/mg	m（Water）/g	w（Solid）/%
RSF	0.32	0	0	0.748	2.28	0	0.68	32
AM	0	1.92	0	0.374	3.42	1.2	4.08	32
RSF/AM	0.11	1.81	0	0.748	3.42	1.2	4.08	32
RSF/AM/OBC	0.1	1.7	3	0.748	3.42	1.2	1.20	32

下载: 导出CSV

表 2 3DS模型的具体参数

Table 2. Specific parameters of 3DS models

Sample	Diameter of print line/mm	Line angle/（°）	Spacing¹⁾/mm	Scaffold			Scaffold porosity/%
Sample	Diameter of print line/mm	Line angle/（°）	Spacing¹⁾/mm	Length/mm	Width/mm	Layers	Scaffold porosity/%
3DS-10	0.6	90	1.2	15	15	10	58.55
3DS-15	0.6	90	1.2	15	15	15	58.55
3DS-20	0.6	90	1.2	15	15	20	58.55
3DS-45.63%	0.6	90	0.9	15	15	10	45.63
3DS-52.17%	0.6	90	1.0	15	15	10	52.17
3DS-64.81%	0.6	90	1.4	15	15	10	64.81
1） The distance between the centers of the lines

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表 3 丝素蛋白基生物墨水的可打印性及对应水凝胶的力学性能

Table 3. Printability of the silk fibroin based bio-inks and the mechanical properties of corresponding hydrogels

Sample	Elastic modulus/MPa	Elongation at break/%	Modulus of compression/MPa	Compressive strength/MPa	Printability
RSF	RSF hydrogels are soft, brittle and virtually non-stretchable		0.0081 ± 0.0002	0.45 ± 0.01	N/A
AM	0.12 ± 0.02	624.30 ± 151.06	0.15 ± 0.01	0.48 ± 0.04	N/A
RSF/AM	0.18 ± 0.01	323.42 ± 69.69	0.21 ± 0.02	0.63 ± 0.01	N/A
RSF/AM/OBC	3.11 ± 0.22	122.53 ± 11.99	1.99 ± 0.23	7.76 ± 0.26	Good

下载: 导出CSV

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