高级检索

  • ISSN 1008-9357
  • CN 31-1633/O6

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

具有螺线表面的聚肽中空柱的制备与结构

焦莉萍 刘艳萍 金锐琦 续文恒 蔡春华 林嘉平

焦莉萍, 刘艳萍, 金锐琦, 续文恒, 蔡春华, 林嘉平. 具有螺线表面的聚肽中空柱的制备与结构[J]. 功能高分子学报,2022,35(6):517-523 doi: 10.14133/j.cnki.1008-9357.20220531001
引用本文: 焦莉萍, 刘艳萍, 金锐琦, 续文恒, 蔡春华, 林嘉平. 具有螺线表面的聚肽中空柱的制备与结构[J]. 功能高分子学报,2022,35(6):517-523 doi: 10.14133/j.cnki.1008-9357.20220531001
JIAO Liping, LIU Yanping, JIN Ruiqi, XU Wenheng, CAI Chunhua, LIN Jiaping. Preparation and Structure of Polypeptide Hollow Disks with Spiral Nanopattern Surface[J]. Journal of Functional Polymers, 2022, 35(6): 517-523. doi: 10.14133/j.cnki.1008-9357.20220531001
Citation: JIAO Liping, LIU Yanping, JIN Ruiqi, XU Wenheng, CAI Chunhua, LIN Jiaping. Preparation and Structure of Polypeptide Hollow Disks with Spiral Nanopattern Surface[J]. Journal of Functional Polymers, 2022, 35(6): 517-523. doi: 10.14133/j.cnki.1008-9357.20220531001

具有螺线表面的聚肽中空柱的制备与结构

doi: 10.14133/j.cnki.1008-9357.20220531001
基金项目: 国家自然科学基金(52073095,51573049)
详细信息
    作者简介:

    焦莉萍(1997—),女,硕士,主要研究方向为聚肽自组装。E-mail:18336586460@163.com

    通讯作者:

    蔡春华,E-mail:caichunhua@ecust.edu.cn

  • 中图分类号: O63

Preparation and Structure of Polypeptide Hollow Disks with Spiral Nanopattern Surface

  • 摘要: 通过聚(γ-苄基-L-谷氨酸酯)-b-聚乙二醇(PBLG-b-PEG)嵌段共聚物聚肽链段的部分酸解脱苄基及与肉桂醇的酯化反应,得到聚(γ-苄基-L-谷氨酸酯-co-肉桂基-L-谷氨酸酯)-b-聚乙二醇(P(BLG/CLG)-b-PEG)嵌段共聚物。肉桂醇基团上的C=C双键在紫外光照射下可发生环加成反应。采用溶液自组装方法,以四氢呋喃-N,N-二甲基甲酰胺(THF-DMF)混合溶剂为起始共溶剂,水为选择性溶剂,P(BLG/CLG)-b-PEG嵌段共聚物与聚苯乙烯(PS)均聚物共混自组装,形成了表面带纳米条纹的核壳型粒子,其中疏水均聚物形成球形内核,聚肽嵌段共聚物形成表面纳米条带。通过改变初始有机溶剂性质,调控了表面纳米条纹的分布形式,得到了经线球和螺线球。通过紫外照射将螺线球和经线球表面条纹中的聚肽链段交联,并用THF溶解PS内核,分别制备了具有表面有序纳米条纹图案的中空柱状和贝壳状粒子。采用扫描电子显微镜(SEM)、透射电子显微镜(TEM)和原子力显微镜(AFM)等测试方法表征聚集体形貌。研究表明:溶剂溶解可以有效去除交联螺线球和经线球的PS均聚物内核,分别制备得到新颖的纳米条纹图案化中空柱状和贝壳状粒子;这些图案化中空柱状粒子结构稳定,保持了3D结构特征;其内部空间具有良好的填充性能,可以被疏水均聚物填充。

     

  • 图  1  P(BLG/CLG)-b-PEG嵌段共聚物的合成路线

    Figure  1.  Synthetic route of P(BLG/CLG)-b-PEG block copolymer

    图  2  嵌段共聚物的1H-NMR图谱

    Figure  2.  1H-NMR spectra of block copolymers

    图  3  中空柱状粒子的制备流程示意图

    Figure  3.  Schematic illustration for the preparation of hollow disk-like particles

    图  4  (a, b)PBLG-b-PEG/PS和(c, d)P(BLG/CLG)-b-PEG/PS自组装聚集体的SEM图

    Figure  4.  SEM images of aggregates formed by self-assembly of (a, b) PBLG-b-PEG/PS and (c, d) P(BLG/CLG)-b-PEG/PS

    图  5  交联后P(BLG/CLG)-b-PEG/PS组装体(a)螺线球和(b)经线球的 SEM 形貌图;中空柱状粒子的(c)SEM图和(d)TEM图;中空贝壳状粒子的(e)SEM图和(f)TEM图

    Figure  5.  SEM images of (a) spiral spheres and (b) meridian spheres of aggregates formed by self-assembly of P(BLG/CLG)-b-PEG/PS after UV-irradiation; (c) SEM and (d) TEM images of hollow disk-like particles; (e) SEM and (f) TEM images of hollow conchoidal particles

    图  6  中空柱状粒子的(a)AFM图和(b)AFM截面分析图

    Figure  6.  (a) AFM image and (b) AFM cross-section analysis of hollow disk-like particles

    图  7  中空柱状粒子的SEM图

    Figure  7.  SEM images of hollow disk-like particles

    图  8  实心柱状粒子的(a)SEM图和(b)示意图

    Figure  8.  (a) SEM image and (b) schematic diagram of solid disk-like particles

  • [1] LI W J, SZOKA F C. Lipid-based nanoparticles for nucleic acid delivery [J]. Pharmaceutical Research,2007,24(3):438-449. doi: 10.1007/s11095-006-9180-5
    [2] MCCORMICK C L, KIRKLAND S E, YORK A W. Synthetic routes to stimuli-responsive micelles, vesicles, and surfaces via controlled/living radical polymerization [J]. Journal of Macromolecular Science Part C,2006,46(4):421-443. doi: 10.1080/15583720600945428
    [3] BLANAZS A, ARMES S P, RYAN A J. Self-assembled block copolymer aggregates: From micelles to vesicles and their biological applications [J]. Macromolecular Rapid Communications,2009,30(4-5):267-277. doi: 10.1002/marc.200800713
    [4] READ E S, ARMES S P. Recent advances in shell cross-linked micelles [J]. Chemical Communications,2007(29):3021-3035. doi: 10.1039/b701217a
    [5] DISCHER D E, ORTIZ V, SRINIVAS G, KLEIN M, KIM Y, CHRISTIAN D, CAI S, PHOTOS P, AHMED F. Emerging applications of polymersomes in delivery: From molecular dynamics to shrinkage of tumors [J]. Progress in Polymer Science,2007,32(8-9):838-857. doi: 10.1016/j.progpolymsci.2007.05.011
    [6] ZHU Y Q, YANF B, CHEN S, DU J Z. Polymer vesicles: Mechanism, preparation, application, and responsive behavior [J]. Progress in Polymer Science,2017,64:1-22. doi: 10.1016/j.progpolymsci.2015.05.001
    [7] LI G Y, YU N N, GAO Y R, TAO Q, LIU X Y. Polymeric hollow spheres assembled from ALG-g-PNIPAM and beta-cyclodextrin for controlled drug release [J]. ChemInform,2016,82:381-386.
    [8] LI H X, BIAN Z F, ZHU J, ZHANG D Q, LI G S, HUO Y N, LU Y F. Mesoporous titania spheres with tunable chamber stucture and enhanced photocatalytic activity [J]. Journal of the American Chemical Society,2007,129(27):8406-8407. doi: 10.1021/ja072191c
    [9] BENTZ K C, EJAZ M, ARENCIBIA S, SULTAN N, GRAYSON S M, SAVIN D A. Hollow amphiphilic crosslinked nanocapsules from sacrificial silica nanoparticle templates and their application as dispersants for oil spill remediation [J]. Polymer Chemistry,2017,8(34):5129-5138. doi: 10.1039/C7PY00342K
    [10] KIM S W, KIM M, LEE W, HYEON T. Fabrication of hollow palladium spheres and their successful application to the recyclable heterogeneous catalyst for suzuki coupling reactions [J]. ChemInform,2002,124(26):7642-7643.
    [11] XUE J X, GUAN Z, LIN J P, CAI C H, ZHANG W J, JIANG X Q. Cellular internalization of rod-like nanoparticles with various surface patterns: Novel entry pathway and controllable uptake capacity [J]. Small,2017,13(24):1604214. doi: 10.1002/smll.201604214
    [12] CARLSEN A, LECOMMANDOUX S. Self-assembly of polypeptide-based block copolymer amphiphiles [J]. Current Opinion in Colloid & Interface Science,2009,14(5):329-339.
    [13] BONDUELLE C. Secondary structures of synthetic polypeptide polymers [J]. Polymer Chemistry,2018,9(13):1517-1529. doi: 10.1039/C7PY01725A
    [14] CAI C H, LIN J P, ZHUANG Z L, ZHU W J. Ordering of polypeptides in liquid crystals, gels and micelles [J]. Advances in Polymer Science,2013,259:159-199.
    [15] LIU Q M, ZHU H S, QIN J Y, DONG H Q, DU J Z. Theranostic vesicles based on bovine serum albumin and poly(ethylene glycol)-block-poly(l-lactic-co-glycolic acid) for magnetic resonance imaging and anticancer drug delivery [J]. Biomacromolecules,2014,15(5):1586-1592. doi: 10.1021/bm500438x
    [16] DEMING T J. Synthetic polypeptides for biomedical applications [J]. Progress in Polymer Science,2007,32(8-9):858-875. doi: 10.1016/j.progpolymsci.2007.05.010
    [17] GHAHREMANKHANI A A, DORKOOSH F, DINARVAND R. PLGA-PEG-PLGA tri-block copolymers as in situ gel-forming peptide delivery system: Effect of formulation properties on peptide release [J]. Pharmaceutical Development and Technology,2008,13(1):49-55. doi: 10.1080/10837450701702842
    [18] DING H, PORTILLA-ARIAS J, PATIL R, BLACK K L, LJUBIMOVA J Y, HOLLER E. The optimization of polymalic acid peptide copolymers for endosomolytic drug delivery [J]. Biomaterials,2011,32(22):5269-5278. doi: 10.1016/j.biomaterials.2011.03.073
    [19] TANG Z M, GAO L, LIN J P, CAI C H, YAO Y, GUERIN G, TIAN X H, LIN S L. Anchorage-dependent living supramolecular self-assembly of polymeric micelles [J]. Journal of the American Chemical Society,2021,143:14684-14693.
    [20] XU P F, GAO L, CAI C H, LIN J P, WANG L Q, TIAN X H. Helical toroids self-assembled from a binary system of polypeptide homopolymer and its block copolymer [J]. Angewandte Chemie International Edition,2020,59:14281-14285. doi: 10.1002/anie.202004102
    [21] LU Y Q, LIN J P, WANG L Q, ZHANG L S, CAI C H. Self-assembly of copolymer micelles: Higher-level assembly for constructing hierarchical structure [J]. Chemical Reviews,2020,120:4111-4140. doi: 10.1021/acs.chemrev.9b00774
    [22] XU W H, XU Z W, CAI C H, LIN J P, GAO L, QI H M, LIN S L. Spiral- and meridian-patterned spheres self-assembled from block copolymer/homopolymer binary systems [J]. Nanoscale,2021,13(33):14016-14022.
    [23] CAI C H, ZHU W J, CHEN T, LIN J P, TIAN X H. Synthesis and self-assembly behavior of amphiphilic polypeptide-based brush-coil block copolymers [J]. Journal of Polymer Science Part A:Polymer Chemistry,2009,47(22):5967-5978. doi: 10.1002/pola.23640
    [24] 王红权, 续文恒, 张朔, 姜翔宇, 李慧, 陈家琛, 蔡春华, 林嘉平. 聚肽HVLPs的制备与载药性能 [J]. 功能高分子学报,2020,33(3):245-252. doi: 10.14133/j.cnki.1008-9357.20190424001

    WANG H Q, XU W H, ZHANG S, JIANG X Y, LI H, CHEN J C, CAI C H, LIN J P. Polypeptide-based hollow virus-like particles: Preparation and drug-loading property [J]. Journal of Functional Polymers,2020,33(3):245-252. doi: 10.14133/j.cnki.1008-9357.20190424001
    [25] 冉海燕, 洪慧, 诸超, 邱野, 吴瑞雪, 朱叶, 罗静, 刘晓亚. 肉桂酸改性透明质酸颗粒乳化剂的制备与性能 [J]. 功能高分子学报,2019,32(1):53-62.

    RAN H Y, HONG H, ZHU C, QIU Y, WU R X, ZHU Y, LUO J, LIU X Y. Preparation and performance of particulate emulsifier self-assembled from cinnamic acid modified hyaluronic acid [J]. Journal of Functional Polymers,2019,32(1):53-62.
  • 加载中
图(8)
计量
  • 文章访问数:  94
  • HTML全文浏览量:  29
  • PDF下载量:  48
  • 被引次数: 0
出版历程
  • 收稿日期:  2022-05-31
  • 网络出版日期:  2022-06-30
  • 刊出日期:  2022-12-01

目录

    /

    返回文章
    返回