Preparation and Photothermal Antibacterial Properties of Zr-Fc MOF@MN Composited Microneedles
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摘要: 将光热性锆-二茂铁基金属有机框架(Zr-Fc MOF)负载到可溶性聚乙烯醇/聚乙烯吡咯烷酮(PVA/PVP)微针(MN)中,开发了一种基于可溶性Zr-Fc MOF负载微针(Zr-Fc MOF@MN)的光热抗菌疗法。采用自下而上法水热合成具有光热性能的Zr-Fc MOF纳米片,并通过X射线衍射,傅里叶红外光谱和扫描电子显微镜对Zr-Fc MOF的结构和形貌进行了表征,并利用红外线热像仪和涂板计数法研究了其光热性能和抗菌性能。进一步通过模板法制备Zr-Fc MOF@MN,并研究其溶解性能和抗菌性能。结果表明:所合成的Zr-Fc MOF光热性能良好,Zr-Fc MOF(0.4 mg/mL)在2.6 W/cm2近红外光照射10 min后温度可上升至57.4 °C,表现出100%的抗菌率;Zr-Fc MOF@MN可在水溶液中溶解,表现出优异的光热抗菌效果(100%)和低溶血性。Abstract: Zirconium-ferrocene-based metal-organic framework (Zr-Fc MOF) with photothermal activities was loaded into soluble microneedle matrix composed of polyvinyl alcohol/ polyvinylpyrrolidone (PVA/PVP) to construct a microneedle (Zr-Fc MOF@MN) for photothermal antibacterial therapy. Zr-Fc MOF nanosheets were synthesized by bottom-up hydrothermal method. The structure and morphology of the Zr-Fc MOF nanosheets were characterized by X-ray diffraction, fourier transform infrared spectroscopy and scanning electron microscopy. Then, the photothermal and antibacterial properties of Zr-Fc MOF nanosheets were studied by infrared thermal imager and plate counting method. Zr-Fc MOF@MN was further prepared by template method, and the solubility and antibacterial properties of the microneedle patch were characterized. The results showed that Zr-Fc MOF nanosheet possessed good photothermal performance, and the temperature of Zr-Fc MOF (0.4 mg/mL) could rise to 57.4 °C after 10 min irradiation with 2.6 W/cm2 near-infrared light (808 nm). The results of antibacterial experiments showed that Zr-Fc MOF (0.4 mg/mL) could kill about 100% bacteria under 2.6 W/cm2 of near-infrared light irradiation for 10 min. The prepared Zr-Fc MOF@MN could be dissolved in water, which showed almost 100% photothermal bactericidal properties and low hemolysis rates.
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图 4 (a,b) MOF在近红外光照射下的温度随时间变化曲线;(c)近红外光(808 nm,2.6 W/cm2)照射下Zr-Fc MOF分散液(ρ500 μg/mL)的热红外图像
Figure 4. (a,b)Time-temperature change curves of Zr-Fc MOF suspensions NIR (808 nm) irradiation; (c) Thermographic images of Zr-Fc MOF concentrations (ρ= 500 μg/mL) during the NIR irradiation (808 nm,2.6 W/cm2).
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