Abstract: Zirconium-ferrocene-based metal-organic framework (Zr-Fc MOF) with photothermal activities was loaded into soluble microneedle matrix composed of polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) to construct a microneedle (Zr-Fc MOF@MN) for photothermal antibacterial therapy. Zr-Fc MOF nanosheets were synthesized by bottom-up hydrothermal method. The structure and morphology of the Zr-Fc MOF nanosheets were characterized by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. Then, the photothermal and antibacterial properties of Zr-Fc MOF nanosheets were studied by infrared thermal imager and plate counting method. Zr-Fc MOF@MN was further prepared by template method, and the solubility and antibacterial properties of the microneedle patch were characterized. The results showed that Zr-Fc MOF nanosheets possessed good photothermal performance, and the temperature of Zr-Fc MOF (0.4 mg/mL) could rise to 57.4 ℃ after 10 min irradiation with 2.6 W/cm² near-infrared light (808 nm). The results of antibacterial experiments showed that Zr-Fc MOF (0.4 mg/mL) could kill about 100% bacteria under 2.6 W/cm² of near-infrared light irradiation for 10 min. The prepared Zr-Fc MOF@MN could be dissolved in water, which showed almost 100% photothermal bactericidal properties and low hemolysis rates.