聚丁内酰胺/壳聚糖电纺纳米蛛网复合纤维膜的制备及性能

曾贝迪; 陈涛; 赵黎明

doi:10.14133/j.cnki.1008-9357.20210603001

聚丁内酰胺/壳聚糖电纺纳米蛛网复合纤维膜的制备及性能

doi: 10.14133/j.cnki.1008-9357.20210603001

曾贝迪^1,,
陈涛^{1, 2, 3, ,},
赵黎明^{2, 3, 4}

华东理工大学1.材料科学与工程学院, 上海市先进聚合物材料重点实验室
2.
中国轻工业生物材料工程重点实验室
3.
恒天生物基材料联合研究院
4.
生物工程学院, 生物反应器工程国家重点实验室,发酵工业分离提取技术研发中心, 上海 200237

基金项目: 上海市自然科学基金项目(21ZR1416000)

详细信息

作者简介:
曾贝迪（1996—），女，硕士生，主要研究方向为静电纺丝。E-mail：betty_zeng03@163.com

通讯作者:
陈涛，E-mail: tchen@ecust.edu.cn；赵黎明，E-mail: zhaoliming@ ecust.edu.cn

中图分类号: TQ340
计量
- 文章访问数: 110
- HTML全文浏览量: 60
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2021-06-03
- 网络出版日期: 2021-10-21

Preparation and Properties of Polybutyrolactam/Chitosan Electrospun Composite Fiber Membranes with Nano-cobweb Structure

ZENG Beidi^1
,,
CHEN Tao^{1, 2, 3
, ,},
ZHAO Liming^{2, 3, 4}

Shanghai Key Laboratory of Advance Polymer Materials, School of Materials Science and Engineering
2.
Key Laboratory of Biobased Material Engineering, China National Light Industry
3.
ECUST- HI-TECH Biobased Material Research Institute
4.
State Key Laboratory of Bioreactor Engineering, School of Biotechnology， East China University of Science and Technology, Shanghai 200237, China

摘要

摘要: 以生物基材料聚丁内酰胺（PBL）和壳聚糖（CS）为原料，考察了PBL/CS的混合液比例以及混合液浓度对纺丝混合液性质及电纺纤维膜的影响，并探讨了所制得电纺复合纤维膜的细胞相容性。结果表明：增加共混物中CS的含量及混合液浓度均会引起纺丝混合液电导率及黏度的增加；当混合液电导率的增加幅度较大时，有利于制得具有纳米蛛网结构的纤维膜；CS的加入抑制了PBL的结晶，制得的纤维结晶度低，甚至为无定形态。MTT实验表明：PBL/CS纤维膜无细胞毒性，可促进细胞增殖，且具有纳米蛛网结构的电纺复合纤维膜的细胞增殖效果更佳。
- 聚丁内酰胺 /
- 静电纺丝 /
- 壳聚糖 /
- 生物基聚合物 /
- MTT
Abstract: Nano-cobweb fibers supported by conventional electrospun fibers are 2D membranes with structure similar to spider webs, for which the average diameter is less than 50 nm and can be potentially applied in the fields of fine filter, sensor, tissue engineering, high performance protective clothing etc. Herein, polybutyrolactamide (PBL) and chitosan (CS), both derived from biomass and are biodegradable, were applied to construct the electrospun composite fiber membranes with nano-cobweb structure. The effects of blending ratio of PBL to CS and the solution concentration on solution properties as well as the fiber morphology and membrane structure were investigated. The cell compatibility of the electrospun composite fiber membranes was also discussed. It is found that the addition of polycationic CS improved the spinnability of PBL/FA solutions and the composite fibers could be continually electrospun. As the proportion of CS was increased, the conductivity and viscosity of the solutions both increased. Varying CS mass fraction in PBL/CS blend from 10% to 30% at fixed solution mass fraction of 8.0%, the solution viscosity increased even more than the conductivity did, and only fiber membranes without nano- cobweb structure were obtained. Increasing solution concentration also increased the solution conductivity and viscosity, but the increase in conductivity was more significant, which proved to be beneficial to electrospinning composite fiber membranes with nano-cobweb structure. When CS mass fraction in PBL/CS blend was 10%, increasing solution mass fraction from 8.0% to 9.0% didn’t help to get nano-cobweb structure. The nano-cobweb structure with breakages was obtained when CS mass fraction was 20% at the solution mass fraction of 10.0%. The composite fiber membranes with perfect nano-cobweb structure and 100% coverage could be electrospun by further increasing CS content and solution mass fraction to 30% and 11.0%, respectively. The results of Fourier transform infrared (FI-IR) spectroscopy and X-ray diffraction (XRD) indicated that the addition of CS inhibited the crystallization of PBL and the composite fibers of low crystallinity and even amorphous. The results of MTT assay confirmed that PBL/CS electrospun composite fiber membranes were noncytotoxic and could promote cell proliferation, especially for those with nano-cobweb structures. Such biobased and biodegradable composite fiber membranes are promising materials in the field of wound-dressing, nerve repairing and regenerative tissue engineering.
- polybutyrolactam /
- electrospinning /
- chitosan /
- bio-based polymer /
- MTT

HTML全文

图 1 PBL/CS纤维膜的形貌：（a～c）纤维膜的SEM照片；（a1～c1）纤维的直径分布图

Figure 1. Morphology of PBL/CS composite fiber membranes: (a—c) SEM images of fiber membranes; (a1~c1) Diameter distribution of fiber

w（CS）/%; a, a1—10; b, b1—20; c, c1—30;w_p=8.0%

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图 2 PBL/CS复合纤维膜的形貌：（a～c） PBL/CS复合纤维膜的SEM照片；（a1～c1）支架纤维的直径分布；（b2，c2）：（b，c），中蛛网纤维的直径分布

Figure 2. Morphology of PBL/CS composite fiber membranes: （a—c） SEM images of samples; （a1—c1） Diameter distribution of samples; （b2—c2） Diameter distribution of nano-cobweb fibers in (b，c), respectively

下载: 全尺寸图片幻灯片

图 3 PBL/CS复合纤维膜的FI-IR谱图

Figure 3. FI-IR spectra of PBL/CS composite fiber membranes

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图 4 PBL/CS复合纤维膜及PBL粉末的XRD图

Figure 4. XRD curves of PBL powder and PBL/CS composite fiber membranes

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图 5 PBL/CS复合纤维膜C2C12细胞活性影响的（a）直接MTT图和（b）间接MTT图

Figure 5. (a) Direct and (b) indirect MTT results of PBL/CS composite fiber membranes cultured with C2C12 cells

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图 6 种植细胞PBL/CS复合纤维膜的体外荧光图

Figure 6. Fluorescence of cell cultured PBL/CS composite fiber membranes in vitro

w_p/%: a—9.0, b—10.0, c—11.0; w(CS)/%: a—10.0, b—20.0, c—30.0

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表 1 w（CS）对混合液及纤维膜性质的影响

Table 1. Influence of w(CS) on the properties of solutions and fiber membranes

w(CS)/%	σ/ (mS·cm⁻¹)	η/ (Pa·s)	D/ nm	P/ %
0	3.71	0.46	—	—
10	4.21	1.85	90	33.7
20	5.14	4.77	72	40.2
30	6.02	9.98	94	53.6
σ: Conductivity of solution; η: Viscosity of solution; D: Average diameter of fiber; P: Porosity of fiber membrane

下载: 导出CSV

表 2 不同混合液浓度对混合液及复合纤维膜性质的影响

Table 2. Effect of w_p on the properties of solutions and fiber membranes

w_p/%	w（CS）/%	σ/ （mS·cm⁻¹）	η /（Pa·s）	D/nm		Cobweb coverage/%	P/%
w_p/%	w（CS）/%	σ/ （mS·cm⁻¹）	η /（Pa·s）	scaffold	cobweb	Cobweb coverage/%	P/%
9.0	10	4.71	1.90	61	—	—	72.3
10.0	20	5.68	4.99	140	13	10	46.1
11.0	30	6.57	10.38	164	10	100	55.1

下载: 导出CSV

表 3 w_p对电导率及黏度增幅的影响

Table 3. Effect of w_p on the increase of solution conductivity and viscosity

w（CS）/%	w_p1/%	w_p2/%	Δw_p/%	Δσ /(mS·cm⁻¹)	$\frac{ {\displaystyle\varDelta\sigma} }{\displaystyle {\sigma}}$/%	Δη （Pa·s）	$\frac{ \displaystyle{\varDelta\eta} }{ {\displaystyle {\eta} } } $/%
10	8.0	9.0	1.0	0.50	11.9	0.05	2.7
20	8.0	10.0	2.0	0.54	10.5	0.22	4.6
30	8.0	11.0	3.0	0.55	9.1	0.40	4.0
Δw_p=w_p2-w_p1, Δσ= σ₂-σ₁, Δη=η₂−η₁

下载: 导出CSV

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