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等离子体辅助纳米涂层的构建及其成骨性能

郭西萌 金莉莉 李春旺 何宏燕 刘昌胜

郭西萌, 金莉莉, 李春旺, 何宏燕, 刘昌胜. 等离子体辅助纳米涂层的构建及其成骨性能[J]. 功能高分子学报. doi: 10.14133/j.cnki.1008-9357.20210330004
引用本文: 郭西萌, 金莉莉, 李春旺, 何宏燕, 刘昌胜. 等离子体辅助纳米涂层的构建及其成骨性能[J]. 功能高分子学报. doi: 10.14133/j.cnki.1008-9357.20210330004
GUO Ximeng, JIN Lili, LI Chunwang, HE Hongyan, LIU Changsheng. Construction and Osteogenic Properties of Plasma-Assisted Nano-Coating[J]. Journal of Functional Polymers. doi: 10.14133/j.cnki.1008-9357.20210330004
Citation: GUO Ximeng, JIN Lili, LI Chunwang, HE Hongyan, LIU Changsheng. Construction and Osteogenic Properties of Plasma-Assisted Nano-Coating[J]. Journal of Functional Polymers. doi: 10.14133/j.cnki.1008-9357.20210330004

等离子体辅助纳米涂层的构建及其成骨性能

doi: 10.14133/j.cnki.1008-9357.20210330004
基金项目: 国家自然科学基金创新群体项目(51621002);国家重点研发计划战略性国际科技创新合作重点专项(SQ2018YF020328)
详细信息
    作者简介:

    郭西萌(1994—),女,硕士,主要研究方向为骨修复生物材料。E-mail:simone.guo1111@gmail.com

    通讯作者:

    何宏燕,E-mail:hyhe@ecust.edu.cn

  • 中图分类号: R318.08

Construction and Osteogenic Properties of Plasma-Assisted Nano-Coating

  • 摘要: 通过氧等离子体处理,在聚对苯二甲酸乙二酯(PET)表面引入羟基,提高了其表面的亲水性。利用静电吸附等方式在PET材料表面先后载入表没食子儿茶素没食子酸酯(EGCG)、纤维黏连蛋白(Fn)和骨形态发生蛋白-2 (rhBMP-2),构建了rhBMP-2/EGCG/Fn有机组装的纳米涂层,改性后的PET表面表现出优异的细胞相容性,rhBMP-2的高效载入、活性维持、以及缓慢释放,赋予了改性表面高诱骨活性和成骨分化能力。

     

  • 图  1  B/E-Fn-PET纳米涂层的制备

    Figure  1.  Preparation of the nano coating B/E-Fn-PET

    图  2  PET氧等离子体处理(a)前(b)后和(c)B/Fn-PET的水接触角

    Figure  2.  Water contact angle of oxygen plasma treatment PET (a) before (b) after and (c) B/Fn-PET

    图  3  各样品的SEM照片

    Figure  3.  SEM images of samples

    图  4  不同纳米涂层表面的AFM表观形貌比较

    Figure  4.  Comparison of AFM surface morphologies on the different surfaces

    图  5  rhBMP-2的释放曲线

    Figure  5.  Cumulative release of rhBMP-2

    图  6  rBMSCs在PET、E-PET和E-Fn-PET表面的黏附铺展

    Figure  6.  rBMSCs morphology on PET, E-PET, and E-Fn-PET

    图  7  rBMSCs在不同PET表面上培养后的细胞增殖行为

    Figure  7.  Proliferation of rBMSCs cells on the surfaces of different PET

    图  8  rBMSCs在各样品中培养7 d后的ALP染色比较

    Figure  8.  ALP staining of rBMSCs on different PET surfaces cultured for 7 d

    图  9  rBMSCs在各样品中培养21后 d的茜素红染色

    Figure  9.  Alizarin red S staining of rBMSCs at 21 d

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出版历程
  • 收稿日期:  2021-03-30
  • 网络出版日期:  2021-06-08

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