Tannic Acid-Assisted Rapid Deposition of Epsilon-Poly-L-Lysine Coating for Antibacterial Application
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摘要: 利用单宁酸（TA）的表面沉积功能，结合TA与ε-聚赖氨酸（Ply）的多重相互作用，在聚二甲基硅氧烷（PDMS）表面制备了TA-Ply功能化涂层，得到TA-Ply修饰的PDMS（PDMS-TA-Ply）。通过X射线光电子能谱仪和接触角测量仪表征了材料的表面化学元素和亲疏水性能；通过蛋白质吸附、细菌黏附、生物被膜形成、细胞毒性等实验评价了PDMS-TA-Ply表面的抗菌/抗污性能和生物相容性。结果表明：PDMS-TA-Ply表面具有良好的抗菌/抗污性能和生物相容性，且表面沉积的Ply量越大，其抗菌/抗污性能越好。Abstract: Polydimethylsiloxane (PDMS) has good thermal stability, biocompatibility and corrosion resistance, and it has been widely used in the field of biomedical materials, i.e., medical catheters. However, the surface of PDMS is highly hydrophobic and susceptible to contamination by bacteria and plasma proteins. In this study, PDMS-TA-Ply surfaces were prepared by the rapid and successive deposition of tannic acid (TA) and ε-Poly-L-lysine (Ply) on the PDMS surface via the solution immersion method. The surface chemical elements and hydrophilic properties of PDMS-TA-Ply surfaces were evaluated by X-ray photoelectron spectroscopy (XPS) and contact angle measurement. The anti-protein adsorption, antibacterial adhesion, anti-biofilm formation and cytotoxicity of PDMS-TA-Ply surfaces were evaluated. The results showed that PDMS-TA-Ply surfaces exhibited good antifouling/antibacterial properties and low cytotoxicity toward L929 mouse fibroblasts. In addition, the PDMS-TA-Ply surface with higher content of Ply showed better antifouling/antibacterial performance.
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Key words:
- ε-poly-L-lysine /
- tannic acid /
- antibacterial /
- surface modification /
- polydimethylsiloxane
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图 1 TA与Ply在PDMS表面快速沉积示意图
Figure 1. Schematic illustration of surface deposition of TA and Ply on PDMS surface
图 2 （a）TA溶液与Ply溶液混后前后的图片；空白PDMS和PDMS-TA-Ply表面的（b）XPS谱图和（c）静态水接触角
Figure 2. （a）Photographs of TA and Ply solutions before and after mixing;（b）XPS patterns and（c）the static water contact angles of the PDMS and PDMS-TA-Ply surfaces（*** represents p<0.001, vs PDMS）
图 3 （a）BSA和（b）FBG在裸金芯片和Au-TA-Ply表面的SPR角偏移曲线以及（c）吸附量
Figure 3. SPR sensorgrams of bare Au and Au-TA-Ply surfaces upon exposure to（a）BSA and （b）FBG, （c）adsorbed amounts（* represents p<0.05, ** represents p<0.01, and *** represents p<0.001, vs bare Au）
图 4 空白PDMS和PDMS-TA-Ply表面与（a，b，c）E. coli和（d，e，f）S. aureus接触4 h后的SEM照片
Figure 4. SEM images of adhered（a, b, c）E. coli and（d, e, f）S. aureus on the pristine PDMS and PDMS-TA-Ply surfaces after 4 h incubation
图 5 空白和改性PDMS表面（a）黏附的E. coli和S. aureus在TSB琼脂板形成的菌落图和（b）黏附的活细菌数量，以及对（c）E. coli和（d）S. aureus的接触杀菌实验（1～3分别代表空白PDMS、PDMS-TA-Ply0.5和PDMS-TA-Ply2）
Figure 5. （a）The formed E. coli and S. aureus colonies and （b）the number of live bacteria on the pristine and modified PDMS surfaces, and the contact killing performance of the pristine and modified PDMS substrates against（c）E. coli and（d）S. aureus（1, 2, and 3 represent pristine PDMS, PDMS-TA-Ply0.5 and PDMS-TA-Ply2 surfaces）
图 6 空白PDMS和PDMS-TA-Ply表面形成的E. coli 和 S. aureus生物被膜的CLSM图像
Figure 6. CLSM images of E. coli and S. aureus biofilms formed on the pristine PDMS and PDMS-TA-Ply surfaces
图 7 空白PDMS和PDMS-TA-Ply表面形成的（a，b，c）E. coli和（d，e，f）S. aureus生物被膜的SEM照片
Figure 7. SEM images of （a, b, c）E. coli and （d, e, f）S. aureus biofilms on the pristine PDMS and PDMS-TA-Ply surfaces
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