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    刘畅, 易兵成, 王先流, 沈炎冰, 秦春萍, 张彦中. Lys-GO对PLCL形状记忆纤维的力学增强和成骨诱导作用[J]. 功能高分子学报, 2020, 33(5): 483-491. doi: 10.14133/j.cnki.1008-9357.20191208001
    引用本文: 刘畅, 易兵成, 王先流, 沈炎冰, 秦春萍, 张彦中. Lys-GO对PLCL形状记忆纤维的力学增强和成骨诱导作用[J]. 功能高分子学报, 2020, 33(5): 483-491. doi: 10.14133/j.cnki.1008-9357.20191208001
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    LIU Chang, YI Bingcheng, WANG Xianliu, SHEN Yanbing, QIN Chunping, ZHANG Yanzhong. Effects of Lysine-Grafted Graphene Oxide on the Mechanical and Osteogenesis Properties of Shape Memory Capable PLCL Fibers[J]. Journal of Functional Polymers, 2020, 33(5): 483-491. doi: 10.14133/j.cnki.1008-9357.20191208001
    Citation: LIU Chang, YI Bingcheng, WANG Xianliu, SHEN Yanbing, QIN Chunping, ZHANG Yanzhong. Effects of Lysine-Grafted Graphene Oxide on the Mechanical and Osteogenesis Properties of Shape Memory Capable PLCL Fibers[J]. Journal of Functional Polymers, 2020, 33(5): 483-491. doi: 10.14133/j.cnki.1008-9357.20191208001
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    Lys-GO对PLCL形状记忆纤维的力学增强和成骨诱导作用

    Effects of Lysine-Grafted Graphene Oxide on the Mechanical and Osteogenesis Properties of Shape Memory Capable PLCL Fibers

      摘要
    • 摘要: 将氧化石墨烯（GO）与具形状记忆特性的乳酸-己内酯共聚物（PLCL）混合电纺制备了GO/PLCL复合纤维。然后将碱性氨基酸—赖氨酸（Lys）通过化学方法与GO共价嫁接得到Lys-GO后电纺引入到PLCL纤维中，形成Lys-GO/PLCL复合纤维，并对其力学性能、形状记忆性能、酸度中和能力以及成骨分化效果等进行了表征和检测。结果表明：将GO引入到PLCL纤维中后，与PLCL膜相比，GO/PLCL纤维膜的杨氏模量提高了28.4%，形状回复力提高了28.3%；而Lys-GO的引入不仅在维持GO/PLCL纤维膜基本性能的前提下显著减缓PLCL的降解导致的酸性问题，而且增强了纤维支架的细胞相容性和成骨分化功效。

       

      Abstract: Graphene oxide (GO) nanosheets with varied mass fractions were introduced into the shape memory capable poly(lactide-co-caprolactone) (PLCL) copolymer to prepare GO/PLCL composite nanofibers via electrospinning. Based on the tensile and shape memory properties of the produced GO/PLCL nanofiber films, an optimal GO mass fraction(m(GO)∶m(PLCL)), 0.5% was determined to maximize the reinforcing effect of the GO nanosheets to the PLCL fiber matrix. Thereafter, an alkaline amino acid lysine (Lys) was selected to functionalize the GO (0.5%) via 1-(3-dimethy laminopropyl)-3-ethylcarbodiimide/N-hydroxysuccinimide chemistry followed by electrospinning for the generation of multifunctional Lys-GO/PLCL nanofibers. A series of characterization including mechanical properties, shape memory performance, acidity neutralization capacity and osteogenic differentiation were subsequently carried out with the produced Lys-GO/PLCL nanofibers. The results showed that among the four groups of GO/PLCL nanofibers loaded with different GO mass fractions (i.e., 0, 0.5%, 1% and 2%), incorporation of 0.5% GO within the PLCL nanofibers gave rise to the most remarkable enhancement efficiency, which was evidenced by 28.4% increase in Young's modulus and 28.3% increase in shape recovery stress. With the introduction of merely 0.5% of Lys-GO into the PLCL nanofibers, it was found that the fiber morphology, tensile properties and shape recovery stress of the GO/PLCL could be largely preserved (>85%). Most importantly, it was demonstrated that the developed Lys-GO/PLCL nanofibers not only enabled neutralize the acidic degradation products of the PLCL (e.g., the pH acidity was neutralized to 5.2 for the Lys-GO/PLCL nanofibers, compared to that of the GO/PLCL counterpart with a pH of 4.2), but also possessed good cytocompatibility and osteogenic differentiation capacity in the mouse bone mesenchymal stem cells (rBMSCs). This newly developed shape memory capable Lys-GO/PLCL fibers may find applications in the construction of multifunctional bone tissue engineering scaffolds.

       

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