Abstract:
Poly(sarcosine-
co-glutamate hydrazide) (P(Sar-
co-GH)) with good water solubility and hydrazide group and oxidized sodium alginate (OSA) with aldehyde group were designed and prepared. Under mild conditions, hydrogels composed of P(Sar-
co-GH), OSA and carboxymethyl chitosan (CMC) could be prepared based on acylhydrazone bonds formed between P(Sar-
co-GH) and OSA and imine bonds formed between CMC and OSA. The structure and mechanical properties of the hydrogels were characterized by FT-IR and rotational rheometer. Results show that the mechanical properties of hydrogels can be adjusted from 840 Pa to 5 690 Pa by changing the content of P(Sar-
co-GH) and CMC. The double dynamic chemical bond hydrogel can be injected by the 23 G needle and also exhibit the self-healing behavior within 12 h. Moreover, the hydrogels can undergo repeated gel-sol transition under the regulation of triethylamine(TEA) and hydrochloric acid(HCl).
In vitro cytotoxicity and cell culture experiments demonstrate that copolymer and hydrogel extract are nontoxic to mouse embryonic cells(NIH/3T3) and can support NIH/3T3 growth and proliferation. Overall, a new hydrogel based on polypeptide and polysaccharide is developed by dual dynamic covalent bond, which endows the material with injectability, self-healing properties and pH responsiveness and has great potential in biomedical field.