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    郑斌, 贺克伍, 程盛, 董华泽, 余永强, 胡进明. 纳米金和荧光DNA的腺苷和钾离子一体化检测[J]. 功能高分子学报, 2020, 33(1): 22-29. doi: 10.14133/j.cnki.1008-9357.20190124003
    引用本文: 郑斌, 贺克伍, 程盛, 董华泽, 余永强, 胡进明. 纳米金和荧光DNA的腺苷和钾离子一体化检测[J]. 功能高分子学报, 2020, 33(1): 22-29. doi: 10.14133/j.cnki.1008-9357.20190124003
    ZHENG Bin, HE Kewu, CHENG Sheng, DONG Huaze, YU Yongqiang, HU Jinming. Multiple Analytes Detection Based on Gold Nanoparticles and Fluorescent Oligonucleotides in One-Pot[J]. Journal of Functional Polymers, 2020, 33(1): 22-29. doi: 10.14133/j.cnki.1008-9357.20190124003
    Citation: ZHENG Bin, HE Kewu, CHENG Sheng, DONG Huaze, YU Yongqiang, HU Jinming. Multiple Analytes Detection Based on Gold Nanoparticles and Fluorescent Oligonucleotides in One-Pot[J]. Journal of Functional Polymers, 2020, 33(1): 22-29. doi: 10.14133/j.cnki.1008-9357.20190124003

    纳米金和荧光DNA的腺苷和钾离子一体化检测

    Multiple Analytes Detection Based on Gold Nanoparticles and Fluorescent Oligonucleotides in One-Pot

    • 摘要: 利用具有不同荧光修饰的寡聚核苷酸(DNA)作为响应基团、纳米金粒子作为淬灭基团构建了多组分“turn-on”传感器,从而达到制备便捷、检测快速且灵敏,同时尽量减少干扰的目的。由于不同检测物识别序列的互补链上修饰的荧光基团不同,可通过不同的荧光达到对不同检测物检测的目的。利用此原理构建了针对腺苷和钾离子的传感器,对腺苷的检出限达387.9 nmol/L,检测范围0~15 μmol/L,通过调节识别序列检测范围可提高到mmol/L级别;对钾离子的检出限达1.6 μmol/L,检测范围2~6 μmol/L。

       

      Abstract: Multiple analytes detection has been the main issue in the field of sensor. To achieve convenient preparation while retain the good sensitivity with little interferences, oligonucleotides (DNA) labelled by fluorescent dyes with different emission wavelengths were adopted as the sensing elements and gold nanoparticles (AuNPs) were selected as a quencher. In the presence of the target in the system, the formation of secondary structure induced by target binding onto aptamer will exclude fluorescent oligonucleotide from binding, releasing a signal-on sensor. Therefore, carboxyfluorescein (FAM) modified DNA adopted as the complementary strand for adenosine detection DNA and rhodamine (ROX) modified DNA complementary for potassium ions recognition DNA have been designed. It was observed that the detection range of adenosine was within 0−15 μmol/L with a detection limit of 387.9 nmol/L with the use of a 20 nmol/L recognition DNA, while the detection range can be enhanced to be 2−15 mmol/L with a recognition DNA concentration of 100 nmol/L. Therefore, the detection range can be simply tuned by changing the number of modified DNA on the surfaces of AuNPs. In the same way, the linear range of potassium ions was determined to be within 2−6 μmol/L and the detection limit was 1.6 μmol/L. Since the fluorescent signals of FAM and ROX present at 520 nm and 605 nm, respectively, the simultaneous detection of multiple analytes could be achieved by simply mixing and simultaneously detecting the fluorescence. The specificity of this method has also been verified using analogues controls. Adenosine could generate a signal with a strength of 5 folds higher than other structurally similar substances, while one third concentration of potassium ions of those analogues could create a signal with a strength of more than 2 folds than other similar ions.

       

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