Abstract:
In order to improve the biological activity and promote the osteogenic differentiation capability of the implanted metallic materials, SLA-Ti was prepared from pure titanium (Ti) through sandblasting and acid etching (SLA). Then, ALN-SLA-Ti was formed by hydrophilic treatment on Ti with alendronate sodium (ALN). Using chitosan (CS) as the main material for the coating, ALN-SLA-Ti surface was coated with CS and immobilized protein by electrostatic spraying (ES). Human bone morphogenetic protein-2 (rhBMP-2) was rapidly and efficiently loaded onto the surface of ALN-SLA-Ti. Coatings loaded with active factors were constructed to enhance the biological activity of titanium surfaces. The morphology of the titanium surface was observed by scanning electron microscope. Bovine serum albumin (BSA) was used as a model protein to evaluate the release behavior
in vitro. The adhesion and proliferation of cells on the surface of titanium was observed by laser confocal microscopy. Result showed that ALN-SLA-Ti formed a microsphere structure after 5 min of ES. The treated surface had multi-level pore structures. Immobilized protein rhBMP-2 was continuously released in a controlled manner. CS coating with rhBMP-2 was conducive to cell adhesion and proliferation, and significantly promote osteogenic differentiation of cells.