Abstract: In order to enhance the hydrophilicity and bioactivity of poly (L-lactic acid) (PLLA), Laponite (LAP) with good hydrophilicity and nanodisked structure was integrated into PLLA nanofiber membranes using an airbrushing technique. The morphology, chemical structure, mechanical properties and hydrophilicity of the nanofiber membranes were characterized by scanning electron microscopy (SEM), Fourier transform infrared analysis (FT-IR), universal testing machine and contact angle measuring instrument. Their biodegradability and cytocompatability were also investigated. It was found that the airbrushing technique allowed for homogeneous introduction of LAP in PLLA matrix to form hybrid nanofiber membranes containing fibers with diameters of 200—300 nm. LAP modification was beneficial to the improvement of the hydrophilicity of the native PLLA nanofiber membranes (with water contact angle changing from 120° for PLLA to 100° for the modified one). Compared to the native PLLA nanofiber membranes, composite nanofiber membranes with 3% LAP (the mass ratio of LAP to PLLA was 3%) presented a 2.5-fold enhancement in tensile strength and a 4-fold increase in Young's modulus. The LAP-modified systems displayed accelerated degradation rate probably due to their hydrophilicity. In vitro biological evaluation displayed that the composite nanofiber membranes could promote the proliferation of C2C12 cells, indicating their potential as a kind of new biomaterial for tissue regeneration.