Abstract:
By using photo-sensitive molecule cinnamic acid as hydrophobic moiety, amphiphilic macromolecule cinnamic acid modified dextran (Dex-CINN) was prepared through the esterification reaction. The structure and substitution degree of Dex-CINN were characterized by Fourier Transform Infrared spectroscopy (FT-IR) and Hydrogen Nuclear Magnetic Resonance (
1H-NMR).The obtained Dex-CINN and template molecule-glucose(Glu) were co-assembled in selective solvent to form molecularly imprinted nanoparticles (MINPs). The size, morphology and Zeta potential of the MINPs were studied by Zeta potential and particle size analyzer and Transmission Electron Microscopy (TEM). The MINPs were first deposited on the surface of the electrode by drop-coating or electrodeposition technique to prepare the MINPs coatings and then followed by UV radiation. After removing the template molecules by extraction, the imprinted MINPs sensor coating were obtained. The morphology of the two kinds of MINPs sensor coatings was studied by Scanning Electron Microscopy (SEM), and their electrochemical performances were evaluated by Cyclic Voltammetry (CV) and Differential Pulse Stripping Voltammetry (DPSV). The results showed that using drop-coating or electrodeposition technique could prepare MINPs sensor coating on the surface of electrode. Compared to the drop-cast coating, the electrodeposition MINPs sensor coating showed good response, selectivity towards glucose and lower detection limit.