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    曲春波, 张静怡, 那立欣, 罗静. 金纳米粒子/聚吡咯-聚多巴胺电化学免疫传感器[J]. 功能高分子学报, 2022, 35(1): 77-84. doi: 10.14133/J.CNKI.1008-9357.20210411002
    引用本文: 曲春波, 张静怡, 那立欣, 罗静. 金纳米粒子/聚吡咯-聚多巴胺电化学免疫传感器[J]. 功能高分子学报, 2022, 35(1): 77-84. doi: 10.14133/J.CNKI.1008-9357.20210411002
    QU Chunbo, ZHANG Jingyi, NA Lixin, LUO Jing. Electrochemical Immunosensor Based on Gold Nanoparticles/Polypyrrole-Polydopamine[J]. Journal of Functional Polymers, 2022, 35(1): 77-84. doi: 10.14133/J.CNKI.1008-9357.20210411002
    Citation: QU Chunbo, ZHANG Jingyi, NA Lixin, LUO Jing. Electrochemical Immunosensor Based on Gold Nanoparticles/Polypyrrole-Polydopamine[J]. Journal of Functional Polymers, 2022, 35(1): 77-84. doi: 10.14133/J.CNKI.1008-9357.20210411002

    金纳米粒子/聚吡咯-聚多巴胺电化学免疫传感器

    Electrochemical Immunosensor Based on Gold Nanoparticles/Polypyrrole-Polydopamine

    • 摘要: 以金纳米粒子功能化的聚吡咯-聚多巴胺(PPy-PDA)为基质材料,构建了电化学免疫传感器用于癌胚抗原(CEA)的检测。首先制备了PPy-PDA复合物,通过聚多巴胺的弱还原性原位还原氯金酸,得到纳米金/聚吡咯-聚多巴胺(Au/PPy-PDA)纳米复合材料。该复合材料具有优异的导电性、水分散性和黏附性,能够在电极表面形成均一、稳定且生物相容性优良的导电膜,利用纳米金与癌胚抗体的特殊作用固定癌胚抗体,并通过牛血清白蛋白屏蔽电极表面的非特异性吸附点,从而构筑了一种用于检测CEA的电化学免疫传感器。所制备的传感器对CEA具有特异性、识别性,在最优条件下,对CEA的线性检测范围为10−12 ~5×10−7 g/mL,检测下限为0.2 pg/mL。此外,还考察了该传感器的重现性和稳定性,并进行了实际样品中CEA的回收实验。该传感器具有检测范围宽、检测限低且稳定性好的特点,在生物医学、临床诊断等方面具有潜在的应用价值。

       

      Abstract: A novel ultrasensitive impedimetric immunosensor was constructed for the detection of carcino-embryonic antigen (CEA) using conductive and adhesive bio-inspired gold/polypyrrole-polydopamine nanocomposites as an immobilization matrix. A polypyrrole-polydopamine (PPy-PDA) complex was first prepared by the polymerization of pyrrole and dopamine, which was then blended with the chloroauric acid solution (HAuCl4). The in-situ reduction of \rm AuCl_4^\;\;- to gold nanoparticles (Au NPs) by polydopamine led to the successful preparation of gold/polypyrrole-polydopamine nanocomposites (Au/PPy-PDA). Au/PPy-PDA was characterized by Fourier transmission infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM) coupled with energy dispersive X-ray spectroscopy (EDX) and electrical conductivity test. The dispersion test and adhesion test showed that PPy-PDA possessed good dispersibility in water and outstanding adhesion performance. The electrochemical measurement showed that Au/PPy-PDA not only provided a highly stable and biocompatible matrix for the convenient conjugation of antibody, but also accelerated the electron transfer process owing to the conductive PPy as well as encapsulated AuNPs, making it suitable to be applied as high-efficiency immunoassay platform. Under optimal conditions, a wide linear range from 10−12 ~5×10−7 g/mL and a detection limit of 0.2 pg/mL were demonstrated for the detection of CEA. Moreover, the proposed CEA immunosensor exhibited high specificity, excellent stability and reproducibility, providing an experimental evidence for early diagnosis and treatment of cancer.

       

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