两种固定化金属螯合复合亲和膜色谱介质制备

Preparation of Two Kinds of Immobilized Metal-chelated Affinity Chromatography Membranes

摘要: 以纤维素滤纸为基质，通过碱处理、环氧活化、偶联亚氨基二乙酸二钠、固定化Ｃｕ＾２＋后制得了大孔纤维素亲和膜。另外，在活化后的膜上通过共价交联覆盖上琼脂糖，制得了具有类似“三明治”结构且性能优于的复合亲和膜，装柱后分别制得固定化金属螯合亲和膜色谱柱。对两种亲和膜进行牛血清白蛋白等温吸附测定显示，两者的最大吸附量分别为１．１７ｍｇ／ｃｍ＾２和１．３０ｍｇ／ｃｍ＾２，与传统的琼脂糖凝胶类介质吸附量相当，表

Abstract: Immobilized metal chelated affinity chromatography membranes using macroporous cellulose filter papers as stationary phase were prepared with activeagent epichorohydrin , coupling iminodiacetic acid and immobilizing Cu 2+ . Novel poly layer sandwi