Abstract: The chrysin surface molecularly imprinted polymers (MIP) were prepared on SiO₂ via surface molecular imprinting technology. The physicochemical properties, specific adsorption, and solid-phase extraction performance of MIP were investigated. The results showed that MIP successfully formed an imprinting layer with good spherical morphology, uniform particle size, and excellent thermal stability. The adsorption capacity of MIP for chrysin was 43.8 mg/g, with an imprinting factor of 2.37. Moreover, the adsorption of chrysin on the MIP followed a pseudo-second-order kinetics and fitted Langmuir isotherm models. In addition, MIP also had good reusability. The molecularly imprinted solid-phase extraction (MISPE) column was prepared using MIP as the packing material. The MISPE column with high performance liquid chromatography (HPLC) could be applied to separate and purify chrysin from the crude extracts of Oroxylum indicum. The prepared MIPs are suitable for the selective separation of chrysin in complex systems.