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    光交联纳米酶葡聚糖水凝胶的制备及应用

    Preparation and Application of Photo Cross-Linked Nanase Dextran Hydrogels

    • 摘要: 通过酯交换反应,将甲基丙烯酸缩水甘油酯(GMA)接枝到葡聚糖(Dex)上,制备出具备光交联性能的甲基丙烯酰化葡聚糖(Dex-MA)。利用“一锅法”将姜黄素(Cur)负载在沸石咪唑酯骨架材料-8(ZIF-8)的框架内,合成具有pH响应性的沸石咪唑酯骨架-8@姜黄素(ZIF-8@Cur)。将ZIF-8@Cur引入Dex-MA溶液中,加入光引发剂I2959并通过紫外光照射引发其光交联,制备出具有良好力学性能和抗菌性能的光交联水凝胶(Dex-ZC)。通过核磁共振氢谱仪、紫外吸收光谱仪、傅里叶红外光谱仪、X射线衍射仪、扫描电子显微镜等对Dex-MA、ZIF-8@Cur和Dex-ZC水凝胶的结构和形貌进行分析,并通过拉伸实验和抑菌圈实验对Dex-ZC水凝胶的力学性能和抗菌性能进行探究。结果表明:ZIF-8@Cur在酸性环境下,释放速率更快,24 h内累积释放率达到(72.98 ± 0.18)%;Dex-ZC水凝胶具有有序的疏松多孔结构和良好的力学性能。抑菌圈实验表明,加入带有正电荷的ZIF-8@Cur,使得Dex-ZC水凝胶对大肠杆菌和金黄色葡萄球菌具有优异的抗菌性能。

       

      Abstract: The methacrylated dextran (Dex-MA) with photo cross-linked property was prepared by grafting glycidyl methacrylate (GMA) onto dextran (Dex) via an ester exchange reaction. Curcumin (Cur) was loaded in the framework of zeolitic imidazolate framework material (ZIF-8) by “one-pot” method, and then the pH-responsive ZIF-8@Cur was synthesized directly. The photo cross-linked hydrogels (Dex-ZC) with good mechanical and antibacterial properties were prepared by introducing ZIF-8@Cur nanase into Dex-MA solution, adding photo initiator I2959 and initiating photo crosslinking through ultraviolet irradiation. The structure and morphology of Dex-MA, ZIF-8@Cur and Dex-ZC were analyzed by nuclear magnetic resonance hydrogen spectrum, ultraviolet absorption spectrum, Fourier infrared spectroscopy, X-ray diffraction and scanning electron microscope. The mechanical properties and antibacterial properties of Dex-ZC were studied by tensile test and bacteriostatic ring test. Results showed that the release rate of ZIF-8@Cur was faster in acidic environment, and the cumulative release amount of Cur reached (72.98 ± 0.18)% within 24 h. Dex-ZC hydrogel had compact porous structure and good mechanical properties, which was caused by the addition of ZIF-8@Cur. The bacteriostatic ring test showed that the addition of positive charge ZIF-8@Cur made Dex-ZC hydrogel have excellent antibacterial activity against E. coli and S. aureus. This study provided more possibilities for the development of photo cross-linked hydrogel dressings on wound healing.

       

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