负载石榴皮提取物的聚乙烯醇基水凝胶伤口敷料的制备与表征

赵中楠; 张兴群; 翟志毫; 何明宇; 夏鑫; 王颖

doi:10.14133/j.cnki.1008-9357.20220922001

负载石榴皮提取物的聚乙烯醇基水凝胶伤口敷料的制备与表征

doi: 10.14133/j.cnki.1008-9357.20220922001

赵中楠^1,,
张兴群^{1, 2},
翟志毫¹,
何明宇³,
夏鑫¹,
王颖^1, ,

1.
新疆大学纺织与服装学院, 乌鲁木齐 830046
2.
东华大学化学化工与生物工程学院, 上海 201620
3.
新疆大学生命科学与技术学院, 乌鲁木齐 830046

基金项目: 国家自然科学基金项目（51863020）；新疆维吾尔自治区自然科学基金面上项目（2021D01C059）；新疆维吾尔自治区天池博士计划项目(042312060)

详细信息

作者简介:
赵中楠（1997—），男，硕士生，主要从事生物医用敷料的研究工作。E-mail: 1040663393@qq.com

通讯作者:
王　颖，E-mail：328532518@qq.com

中图分类号: R318.08;TB34
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-22
- 网络出版日期: 2022-12-18
- 刊出日期: 2023-04-01

Preparation of Polyvinyl Alcohol Hydrogel Wound Dressing Loaded With Pomegranate Peel Extract

1.
College of Textile and Clothing, Xinjiang University, Urumqi 830046, China
2.
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China
3.
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China

摘要

摘要: 以聚乙烯醇（PVA）和羧甲基纤维素钠（Na-CMC）为原料，以丙三醇（GL）水溶液为溶剂，利用循环冻融法制备一种负载石榴皮提取物（PPE）的水凝胶。对水凝胶的化学结构、透光率、抗干燥性、溶胀性、拉伸性能、体外药物释放、抗氧化性及抗菌性进行了研究。结果表明，制备的水凝胶具备较好的抗干燥性，室温下放置72 h仍能保持完整。PPE的加入使水凝胶的透光率降低，溶胀性、拉伸性提高，同时使水凝胶具备良好的抗菌性和抗氧化性。经测试水凝胶中PPE的累计释放率最高可达（67.47±3.75）%，对金黄色葡萄球菌、大肠杆菌均具有较好的抑菌性，抑菌圈最大分别为（18.78±0.26）mm、（15.11±0.23）mm，对1,1-二苯基-2-苦肼基（DPPH）的清除率最高可达（93.65±2.27）%。
- 石榴皮提取物 /
- 聚乙烯醇 /
- 羧甲基纤维素钠 /
- 水凝胶 /
- 伤口敷料
Abstract: In order to develop a wound dressing loaded with pomegranate peel extract (PPE), the hydrogel was prepared by cyclic freeze-thaw method using polyvinyl alcohol (PVA) and sodium carboxymethyl cellulose (Na-CMC) as raw materials, glycerol (GL) aqueous solution was used as the solvent. Subsequently, the chemical structure of hydrogels was analyzed by FT-IR. In addition, the transmittance, anti-drying, swelling, tensile properties, in vitro drug release, anti-oxidation and antibacterial properties of hydrogels were also studied. Results showed that the prepared hydrogels had good drying resistance and could remain intact at room temperature for 72 h. The addition of PPE reduced the light transmittance of the hydrogel, improved the swelling and tensile property of the hydrogel, and made the hydrogel have good antibacterial and antioxidant properties. The cumulative release of PPE in the hydrogel was (67.47 ± 3.75)%, which showed good antibacterial activity against Staphylococcus aureus and Escherichia coli. The inhibition zones were (18.78 ± 0.26) mm and (15.11 ± 0.23) mm, respectively. The highest scavenging rate to 1,1-diphenyl-2-picrylhydrazyl (DPPH) was (93.65 ± 2.27)%. Therefore, the hydrogel loaded with PPE prepared in this study has the potential as a wound dressing.
- pomegranate peel extract /
- polyvinyl alcohol /
- sodium carboxymethyl cellulose /
- hydrogel /
- wound dressing

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图 1 PPE及水凝胶的红外谱图

Figure 1. FT-IR spectra of PPE and hydrogels

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图 2 水凝胶的透光率

Figure 2. Transmittance of hydrogel

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图 3 （a）24 h与72 h后水凝胶的余重比；（b）72 h后水凝胶的SEM图

Figure 3. (a) Residual mass ratio of hydrogels after 24 h and 72 h; (b) SEM images of the hydrogels after 72 h

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图 4 水凝胶的溶胀性能分析：（a）溶胀动力学曲线；（b）Na-CMC含量对水凝胶最大溶胀率的影响；（c）9 h与72 h后水凝胶的SEM图

Figure 4. Swelling property analysis of hydrogels：(a) Swelling dynamic curves; (b) Effect of NA-CMC content on the maximum swelling ratio of hydrogel; (c) SEM images of hydrogels after 9 h and 72 h

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图 5 水凝胶的（a）拉伸强度和（b）断裂伸长率

Figure 5. (a) Tensile strength and (b) elongation at break of hydrogel

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图 6 水凝胶的体外模拟释放曲线

Figure 6. Simulated release curves in vitro of hydrogels

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图 7 水凝胶的体外抗氧化性能

Figure 7. In vitro antioxidation of hydrogels

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图 8 水凝胶的抑菌性能：（a）实验结果图；（b）抑菌圈直径

Figure 8. Antibacterial properties of hydrogels：(a) Experimental results; (b) Bacteriostatic diameters

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图 9 细菌荧光图及SEM图：（a） S. aureus ；（b）E.coli

Figure 9. Bacterial fluorescence and SEM images: (a) S. aureus; (b) E.coli

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