Abstract: As a potential substitute of surgical suture for wound closure use, biomedical/tissue adhesives can be widely used in the repair/regeneration of wounds on soft/hard tissue such as skin, viscera, cardiovascular, bone and teeth, possessing a broad market prospect. However, it is still a challenge for the science community to produce strong adhesion to tissues at wet state. To address this problem, scientists developed a series of biomimetic tissue adhesives by imitating the adhesion strategies of various animals and plants in nature. In this review, the development of various polymeric biomimetic medical/tissue adhesives and their applications in surgical wound adhesion, daily wound care, chronic wound regeneration, bone fracture fixation and other soft/hard tissue repair/regeneration, as well as local drug delivery and in situ therapy are summarized in detail. The future development trend of tissue adhesives is also prospected, including their application potential in the field of medical cosmetology.