Abstract: Glucosesensitive polyacrylamide hydrogel film was made by incorporating polystyrene latex particles functionalized with perylene diimide. Monodispersity and morphology of latex particles were analyzed with SEM and particle size analyzer. The amount of perylene diimide on the surface of particles was measured by atomic absorption spectrum. Study on fluorescence property of hydrogel film shows that fluorescence intensity of the hydrogel film decreases after the film is immersed in glucose solution, but the emission peak doesn't shift. Fluorescence quenching efficiency is 0.34 in the 200 mmol/L of glucose solution.