Abstract: A series of hybrid fluorescent nanoprobes (DPN@HNP) encapsulating hydrophobic napthali-mide derivatives N-dodecyl-4-piperidyl-1, 8-napthalimide(DPN) were prepared based on the self-assembly of amphiphilic block copolymer polystyrene-b-poly(acrylic acid)(PS-b-PAA) and the hydrolysis of organic silane 3-mercaptopropyltrimethoxy-silane (MPTMS). The influences of the loading amounts of DPN, block copolymer and organic silane on the photophysics properties and morphology of the as-synthesized DPN@HNP were comprehensively investigated. Results show that the absorption and emission spectra of DPN@HNP intensifies and red-shifts with the increase of loading amount and the degree of packing of DPN. The length of PS segment and adding amount of PS-b-PAA have a significant effect on the loading amount and the efficiency of DPN. Both longer PS segment and higher adding amount lead to higher loading amount of DPN and larger diameter of DPN@HNP, thus enhance the emission. The adding amount and reaction time of MPTMS have negligible effects on the photophysics properties of DPN@HNP, while the hybrid silica shell thicken with the increase of the adding amount of MPTMS.