胆红素分子表面印迹材料MIP-PMAA/SiO2的制备及识别特性

Preparation of Molecular Imprinted Material MIP-PMAA/SiO2 and Binding Properties for Bilirubin

摘要: 通过γ-（甲基丙烯酰氧）丙基三甲氧基硅烷的媒介作用，在硅胶微粒表面偶合接枝聚甲基丙烯酸(PMAA)，制备了PMAA/SiO2接枝微粒。采用新型分子表面印迹技术，以乙二醇二缩水甘油醚（EGDE）为交联剂，制备了以胆红素为模板分子的表面印迹材料MIP-PMAA/SiO2。分别通过静态和动态吸附实验考查了该印迹材料对胆红素的识别特性。结果表明：该印迹材料对胆红素兼具优良的结合亲和性和特异的识别选择性，同时，具有优良的脱附性能，脱附率可达99.5%。

Abstract: By coupling action of 3-methacryloxypropyl trimethoxysilane (MPS), polymethylacrylic acid (PMAA) was grafted onto the surface of silica particles. Using novel molecular surface imprinted technique, the molecular imprinted polymer MIP-PMAA/SiO2 was obtained using bilirubin as the template molecular and ethylene glycol diglycidyl ether (EGDE) as the crosslinking agent. Both static and dynamic binding properties of MIP-PMAA/SiO2 for bilirubin were studied. Results show that MIP-PMAA/SiO2 exhibits excellent binding affinity and specific selective recognition to bilirubin. At the same time, this material exhibits excellent desorption performance, and the desorption ratios of bilirubin reach 99.5%.