Abstract: A series of hypercrosslinked porous polymer materials with high specific surface area (HCP-PhH-3, HCP-PhH2-3, HCP-PhH3-3) for tryptophan adsorption were synthesized by Friedel-Crafts alkylation reaction with benzene, biphenyl and 1,3,5-triphenylbenzene as the reaction monomer, dimethoxymethane as the crosslinking agent and 1,2-dichloroethane as the reaction solvent. The structure of the hypercrosslinked porous polymer materials were characterized by nitrogen adsorption and desorption analyses, thermal weight loss and infrared spectroscopy. The BET results showed that the hypercrosslinked porous polymer materials exhibited extremely rich steric pore size structure, such as the specific surface area of HCP-PhH-3 up to 901.50 m²/g, and thermogravimetric analysis of these cross-linked polymers showed that they exhibited good thermal stability. The adsorption behavior of HCP-PhH-3, HCP-PhH2-3 and HCP-PhH3-3 on tryptophan were studied by adsorption method. The experimental results showed that the order of adsorption amount of tryptophan was as follows: HCP-PhH-3 > HCP-PhH2-3 > HCP-PhH3-3. The polymer material synthesized with benzene as monomer showed the greatest adsorption capacity for tryptophan. After immersion in five different acidic and alkaline solvents, the polymers still showed good adsorption of tryptophan, indicating that the hypercrosslinked porous polymer materials had excellent performance in carrying out adsorption processes in different environments. The kinetic fitting and thermodynamic fitting of tryptophan adsorption by HCP-PhH2-3 and HCP-PhH3-3 showed that the adsorption of tryptophan by the polymers were heat-absorbing process and chemical reaction rate-controlled multilayer adsorption process. Finally, the possible relationship between the structures of HCP prepared with different monomers and the adsorption capacity of tryptophan were also explored. This class of hypercrosslinked porous polymer materials offered new options for material applications in the field of amino acid adsorption and separation due to their simple synthesis process and excellent stability and relatively good tryptophan adsorption capacity.